She was born in Montevideo on June 21, 1865. Her parents were Pedro Gallo and Matilde Poggio. She began her piano studies as a child. At the age of nine she traveled to study at the Naples Conservatory, where she was taught by the Italian composer Beniamino Cesi. In 1878, she won the First Grand Prize of the Conservatory, for which she gave a concert at the Teatro Príncipe Amadeo.

Upon her return to Montevideo, in 1878, she gave a concert at the Teatro Solís. A few years later, in 1885, she met the Italian pianist Camilo Giucci, whom she married that same year. Camilo and Luisa had nine children, two of whom died at an early age. Camilo, Luisa, Mario, Carlos, and Esther were involved in various ways to music creation and instruction.

In 1895, Luisa and Camilo founded the Franz Liszt Conservatory of Music, an institution that became a benchmark for music instruction in the country. Luisa played a prominent role at the Conservatory of Music, taking on a piano class that she held until a few years before she passed away. In addition, she performed on numerous occasions as part of concerts and evenings organized by the Conservatory. She thus played a prominent role as a concert pianist, appearing both as a solo artist and as an accompanist.

Luisa Gallo passed away on September 29, 1945.